Projects & Grants
| Protist biology in the 21st century: utilizing functional genetics and bioinformatical analyses for broadening knowledge about protist virulence and metabolism | |
|---|---|
| Project Id | SGS04/PŘF/2025 |
| Main solver | Mgr. Jiří Pergner, Ph.D. |
| Period | 1/2025 - 12/2025 |
| Provider | Specifický VŠ výzkum |
| State | solved |
| Anotation | The proposed project aims to expand understanding of the biology and diversity of parasitic and non-parasitic protist organisms using modern molecular biology techniques such as genome editing using the CRISPR/Cas9 system, differential transcriptomics, detection of cytotoxic effects of substances on microalgae using flow cytometry, and bioinformatics analyses. The research plan reflects the diversity of topics addressed by the principal investigator and co-investigators, including: 1. characterisation of virulence factors in parasitic protozoa of the genus Leishmania; 2. molecular biological analysis of protozoa of the genus Novymonas; 3. characterisation of biochemical processes in parasitic and non-parasitic protozoa of the family Trypanosomatidae; 4. Study of the secondary plastid in the model organism Euglena gracilis; 5.Investigation of genome damage in microalgae following pesticide application. Most components of the project are focused on fundamental research. However, some aspects have the potential to lay the groundwork for future applied research. The project timeline is aligned with the progression of dissertation research topics. The results of the project will be published in renowned peer-reviewed journals with an impact factor and presented at professional conferences. Given the significant involvement of international students (8 out of 10 co-investigators), individual sections of the project are presented in English. |
